Introduction

ARLAB-LCMS2828 Liquid chromatograph - triple quadrupole mass spectrometer, integrating the high separation efficiency of liquid chromatograph and the strong identification capability of mass spectrometer, has sufficient sensitivity and selectivity, good stability and strong anti-interference. Therefore, it is applicable to the qualitative and quantitative analysis on high sensitivity of trace pesticides and veterinary drugs in complex matrix.

Work Environment

1.Work environment temperature

18-25 °C

2.Humidity in work environment

(20 ~ 60)% RH

3.Power supply

five sets of single-phase (220 ± 20) V AC, 10A, 50 Hz power supply

Workstation software:

1. Basic features of software system: Windows XP or above operating system. The software can control the liquid chromatograph and mass spectrometer, with built-in data processing and report editing functions. Besides, the software can realize the function configuration and condition optimization of the instrument automatically, the atomatic quantification, the mass spectrometry data analysis, and the establishment and retrieval of spectral database.

2. The system has the functions of automatic correction and instrument condition monitoring.

3. The LC-MS operation software can be installed on the personal computer, and used for the offline processing of sample analysis data and report generation.

Applications

Detection of sulfonamides in pork
Detection of carbamate pesticides in water
Detection of perfluorinated compounds in environmental media
Screening of neonatal genetic metabolic diseases
Determination of vitamin D in serum

1. Ultra-high pressure gradient pump

Two solutions in A and B & C and D, can be selected as the flowing phase of the system by switching the solvent selection valve. A vacuum degasser is equipped, with each pump degassing separately (A/B).

Flow range

0.001-4.00 ml/min

Maximum pressure

≥ 18,850psi pH range 1-14

Accuracy of flow rate

≤0.1%

Precision of flow rate

≤ 0.075% RSD

2. Automatic sample injector

Injection Volume

1-50ul/min

Sample Cooler temperature setting range

4 °C to 45 °C

Injection Precision

Full loop injection <0.3% RSD; partial loop injection <0.3% RSD;
microliter pickup <0.5% RSD

Carry Over

<0.001%

Maximum sample capacity

384 Vials, and 96 Vials for standard liquid injection.

3. Column oven

Temperature control range

Room temperature -5 °C to 90 °C (Stability & Accuracy ±0.1 Degree C)

Temperature control mode

Preheating of flowing phase + forced air circulation

Maximum column capacity

Six 250mm long chromatographic columns can be installed at the same time .

PDA detector

190-900nm ,512 element, Linearity -2.5AU with 100HZ rate

1. Ion source (ESI & APCI ion source for standard configuration)

Orthogonal vertical spray design is adopted, so that the system has strong anti-pollution capacity and low background noise.

Ion source gas supply

1 loop of nitrogen for atomizing and 2 loops of nitrogen for desolvation. Specifically, the flow rate of nitrogen for atomizing is 0-2 L/min.

Desolvation temperature 650°C in ESI & APCI. The maximum spray voltage is 6kV.

The flow rate, voltage and temperature can be set up and run under the software interface, to ensure the maximum ionization efficiency and resistance to matrix interference.

A special exhaust device is set in the ion source to prevent the backflow of gas in the closed ion source cavity, further to reduce the memory effect and pollution of the ion source, decrease the load of the mechanical pump, prolong the service time of the mechanical pump oil, maintain the test environment and ensure the health of the staff.

2. Vacuum interface and ion transmission system

High temperature blowback gas design

Nitrogen is used as the blowback gas, with a flow rate of 0-5L/min to further remove the solvent and reduce the introduction of neutral molecules .

Heating design of vacuum interface

It can be heated to 110 °C at most to improve the anti-pollution capacity.

Vacuum interface maintenance

simple cleaning and maintenance, without vacuum unloading.

The whole process of daily maintenance and installation can be easily completed in a few minutes.

Ion transmission system

4-stage differential vacuum design is adopted, and multiple quadrupole transmission is used for accurate focusing of ions.

3. Mass analysis system

Mass analyzer

Triple quadrupole mass analyzer.

Quadrupole

Made of high-precision pure Mo material, capable of ensuring the best mass axis stability.

Collision cell

Axial acceleration design is adopted, capable of effectively eliminating the interference of ion pair and ensuring the high-throughput analysis capability. Nitrogen (with purity ≥99.999%) is used as the collision gas and supplied in cylinder.

Cross Talk

0.0005%

MRM Dwell Time

0.8ms

Resolution

< 0.7 ± 0.1 amu (adjustable)

Mass stability

0.05 Da/24 hours.

Scan speed

22000 amu/s

Number of MRM channels (Scan Speed)

555 channels/s

Mass range m/z

5-2000 Da

Dynamic range

7 orders

Sensitivity

ESI+, MRM mode

1pg reserpine, injected on the column, with S/N≥2000000:1, and IDL- 0.2fg

ESI-, MRM mode

1pg chloramphenicol, injected on the column, with S/N ≥ 2000000:1; and IDL- 0.2fg

Scanning functions

Full scan, selected ion monitoring (SIM), product ion scan, precursor ion scan, neutral loss scan, multiple reaction monitoring (MRM), positive/negative ion switching scan, etc.

4. Detector

Electron Multiplier technology (EMT), without any positive and negative ion discrimination effect, which has long service life, capable of ensuring the long-term data stability.

Pulse-counting detector, to ensure the data reproducibility of low limit of detection.

Positive/negative polarity switching time

≤ 10ms

Vacuum system

composed of a mechanical pump and a turbo molecular pump, with a differential pumping system formed between the ion transmission area and mass analysis area, functioning for automatic power-off protection.